去除高丰度蛋白质后水牛卵泡液的双向电泳差异分析

doi:10.11843/j.issn.0366-6964.2014.07.012

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (7): 1113-1119.doi: 10.11843/j.issn.0366-6964.2014.07.012

去除高丰度蛋白质后水牛卵泡液的双向电泳差异分析

黄愉淋¹，付强¹，黄德伦¹，陈富美¹，黄凤玲¹，梁贤威^2*，张明^1*，卢克焕¹

（1.广西大学动物繁殖研究所，亚热带农业生物资源保护与利用国家重点实验室，南宁 530004；2.中国农业科学院广西水牛研究所，南宁 530001）

收稿日期:2013-12-27 出版日期:2014-07-23 发布日期:2014-07-23
通讯作者: 梁贤威，研究员，博士生导师，E-mail：Liangbri@126.com；张明，教授，E-mail：mingzhang@gxu.edu.cn
作者简介:黄愉淋（1988-），女，壮族，广西大化县人，博士，主要从事动物蛋白质组学研究；E-mail：huangyulin1016@163.com
基金资助:
广西水牛遗传繁育重点实验室开放课题（SNKF-2013-02）；国家自然科学基金（30860192）

2-DE Differential Analysis of ALB/IgG Removal Buffalo Follicular Fluid

HUANG Yu-lin¹，FU Qiang¹，HUANG De-lun¹，CHEN Fu-mei¹，HUANG Feng-ling¹，LIANG Xian-wei^2*，ZHANG Ming^1*，LU Ke-huan¹

（1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources，Animal Reproduction Institute， Guangxi University，Nanning 530004，China； 2.Buffalo Research Institute，Chinese Academy of Agricultural Sciences，Nanning 530001，China）

Received:2013-12-27 Online:2014-07-23 Published:2014-07-23

摘要/Abstract

摘要：

以广西沼泽型水牛卵泡液为研究对象，用试剂盒免疫去除成熟及未成熟水牛卵泡液高丰度蛋白质后，采用已建立并优化的双向电泳体系对卵泡液蛋白质样品进行分离。软件分析发现，21个差异表达蛋白质与成熟卵泡液相比，10个蛋白质点表达上调，7个蛋白质点表达下调，3个蛋白质点缺失，1个蛋白质点在水牛未成熟卵泡液中特异性表达。经质谱分析，最终鉴定出3个蛋白质点：硫酸化糖蛋白、转铁蛋白和触珠蛋白。该研究成功分离并鉴定的水牛卵泡液差异蛋白质，为揭示水牛卵母细胞发育的微环境及其成熟机制提供试验依据。其中转铁蛋白对于水牛卵泡液的发育成熟具有十分重要的促进作用，而其它2个蛋白质对水牛卵泡液的成熟相关机制还尚未清楚，有待于进一步的试验研究。

Abstract:

This study employed swamp buffalo follicular fluids in Guangxi as the research object.First describes the purification of mature and immature buffalo follicular fluid samples using a ProteoExtract^TM Albumin/IgG Removal Kit，it then described the isolation of proteins from these purified mature and immature follicular fluids using the established and optimized 2DE system.Twenty-one different expression protein spots were found with Image Master 2D platinum software，10 of these proteins were up-regulated in immature buffalo follicular fluid，7 proteins were down-regulated，3 proteins deleted and 1 protein specifically expressed.Three proteins were successfully identified by mass spectrometry，they were probably sulfated glycoprotein-2，transferrin and HP protein.In conclusion，a group of different expression proteins from mature and immature buffalo follicular fluids were successfully isolated and identified to provide experimental basis for developmental microenviroment and maturation mechanism in buffalo oocytes.And transferrin played an important role in promoting the maturation of buffalo follicular fluid，while the maturation mechanism of other two proteins in buffalo follicular fluid was still not clear and it needs to be further studied.

中图分类号:

S823.8⁺3.2

黄愉淋，付强，黄德伦，陈富美，黄凤玲，梁贤威，张明，卢克焕. 去除高丰度蛋白质后水牛卵泡液的双向电泳差异分析[J]. 畜牧兽医学报, 2014, 45(7): 1113-1119.

HUANG Yu-lin，FU Qiang，HUANG De-lun，CHEN Fu-mei，HUANG Feng-ling，LIANG Xian-wei，ZHANG Ming，LU Ke-huan. 2-DE Differential Analysis of ALB/IgG Removal Buffalo Follicular Fluid[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1113-1119.

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去除高丰度蛋白质后水牛卵泡液的双向电泳差异分析

2-DE Differential Analysis of ALB/IgG Removal Buffalo Follicular Fluid

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